電子對焦成像系統——eTFMS3
系統構成：
LED (465 nm)光源、顯微鏡驅動器、自聚焦顯微鏡、成像套管、換向器、顯微鏡架、虛擬顯微鏡、軟件、電纜套件等。
主要特征：
1）電子調焦：軟件控制的自動調焦，無需手動調節，視野范圍更加穩定
2）最新一代的設計，較少的熒光激發光，減少光漂白和光毒性
3）連接簡單：顯微鏡主體通過旋轉連接到成像套管， 無需任何工具。
4）自由移動：標配Doric獨有的高品質換向器設備，可在各種行為操作箱、迷宮、曠場中移動。
5）單細胞分辨率下，可同時捕獲1000+神經元活動
6）長時間跟蹤細胞，追蹤時間可長達數月
7）散熱快：外接式光源，不存在攝像頭散熱發燙損傷動物
8）模塊化設計，便于攜帶和維修
結合光遺傳的電子對焦成像系統——eTOSMS3
電子對焦+鈣離子成像+光遺傳一體化系統
系統構成：
LISER??+ LED （470nm)?光源和驅動器、電子對焦顯微鏡、成像套管、換向器、顯微鏡架、虛擬顯微鏡、軟件、電纜套件等
主要特征：
1）Ca2+成像和光刺激（激發/抑制）可同步進行
2）鈣離子探針+光遺傳視蛋白
3）生物干擾最小化，信噪比最大化
4）同步跟蹤和行為學分析
頭戴雙色微型熒光顯微鏡系統——2CMS
微型熒光雙色顯微鏡提供了在自由行為動物上可視化大規模神經環路動力學的端到端的解決方案。雙色熒光顯微鏡主體結合了兩個 CMOS 傳感器，可同時對兩種不同的熒光團進行成像?？赏瑫r記錄紅色（如：RFP）和綠色（如：GFP) 熒光標記。與單色微型熒光顯微鏡體不同的是，雙色顯微鏡體針對特定的成像深度進行了優化，成像深度可達 5.9 毫米的大腦區域。
系統構成：
YAG + LED (465 nm) 光源、顯微鏡驅動器、雙色熒光顯微鏡 、成像套管、換向器、顯微鏡支架、虛擬顯微鏡、軟件、電纜套件等。
部分已發表文獻：
?1.?S. Malvaut et al. Live imaging of adult neural stem cells in freely behaving mice using mini-endoscopes?
? ? ??Star Protocols, (2021). |
?2.? B.T. Lain et al. Fluorescence microendoscopy for in vivo deep-brain imaging of neuronal circuits.?
? ? ??Journal of Neuroscience Methods, (2021). |
?3.? J.N. Siemian. An excitatory lateral hypothalamic circuit orchestrating pain behaviors in mice.
? ? ? eLife (2021)
? ? ? Brain region:?Lateral Hypothalamus (LH) |
?4.? A. Gengatharan et al. Adult neural stem cell activation in mice is regulated by the day/night cycle and intracellular calcium dynamics.?
? ? ??Cell 184, 709–722 (2021). |
?5.? F. Fredes et al. Ventro-dorsal Hippocampal Pathway Gates Novelty-Induced Contextual Memory Formation.
? ? ??Current Biology 31, 25–38.e5 (2021)
? ? ? Brain region:?Ventral hilus; dorsal dentate gyrus |
?6.? D. Rossier et al. A neural circuit for competing approach and defense underlying prey capture.
? ? ??PNAS (2021)
? ? ? Brain region:?Lateral Hypothalamus (LHA) |
?7.? A. Glas et al. Spaced training enhances memory and prefrontal ensemble stability in mice.
? ? ? bioRXiv (2020)
? ? ? Brain region:?dorsomedial prefrontal cortex (dmPFC) |
?8.? P. Krzywkowski et al. Dynamic encoding of social threat and spatial context in the hypothalamus.
? ? ??eLife 9, e57148 (2020)
? ? ? Brain region:?Hypothalamus (VMHvl) |
?9.? C. Prévost-Solié et al. Superior Colliculus to VTA pathway controls orienting behavior during conspecific interaction.
? ? ??bioRXiv (2019)
? ? ? Brain region:?Superior Colliculus (SC) |
10.?A. Glas et al. Benchmarking miniaturized microscopy against two-photon calcium imaging using single-cell orientation tuning in mouse ???? ?visual cortex?
? ? ??Plos One (2019)
? ? ? Brain region:?visual cortex (V1) |
11.?J.M. Patel et al. Sensory perception drives food avoidance through excitatory basal forebrain circuits.
? ? ??eLife 8, e44548 (2019)
? ? ? Brain region:?basal forebrain (BF) |
12.?Fu et al. SatB2-Expressing Neurons in the Parabrachial Nucleus Encode Sweet Taste
? ? ??Cell reports 27 , 1650-1656 (2019)
? ? ? Brain region:?parabrachial nucleus (PBN) |
13.?B. Roberts et al. Ensemble encoding of action speed by striatal fast-spiking interneurons?
? ? ??Brain structure and function (2019)
? ? ? Brain region:?dorsal striatum (DS) |
14.?S. Shin et al. Drd3 Signalling in the Lateral Septum Mediates Early Life Stress-Induced Social Dysfunction
? ? ??Neuron 97, 195–208 (2018)
? ? ? Brain region:?lateral septum (LS) |
15.?E. Gallo et al. Accumbens dopamine D2 receptors increase motivation by decreasing inhibitory transmission to the ventral pallidum.
? ? ??Nature Communication 9, 1086 (2018)
? ? ? Brain region:?nucleus accumbens (NAc) |
16.?D.A. Evans et al. A synaptic threshold mechanism for computing escape decisions
? ? ??Nature 558 , 590–594 (2018)
? ? ? Brain region:?dorsal periaqueductal gray (dPAG); medial superior colliculus(mSC) |
17.?T.C. Francis et al., Molecular basis of dendritic atrophy and activity in stress susceptibility.?
? ? ??Molecular psychiatry 22, 1512–1519(2017)
? ? ? Brain region:?nucleus accumbens (NAc) |